Coding

Part:BBa_K3515012

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-06-16)


Parathyroid Hormone Receptor with cysteine modification(s) to bind to a biosensor.

The parathyroid hormone receptor (PTHR) selectively binds parathyroid hormone (PTH) in its N- terminal extracellular domain. A fluorophore may be attached to the N- terminal domain as well to create a FRET signal. This makes it a distinguishable candidate for PTH monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with a supply of PTH bound to a second fluorophore can create a competition assay, where PTH and PTH-fluorophore compete with one another for PTHR. PTH detection is vital as PTH directly regulates renal function and is also a primary regulator of calcium levels. PTH is also a crucial biomarker used in clinical medicine for tracking the progression and status of Chronic Kidney Disease (CKD) patients. As such a biosensor for PTH tracking may be of great interest to patients and clinicians. This part includes the N- terminal extracellular domain of PTHR with a cysteine residue that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of PTH.

File13.png

Cysteine immobilization modifications of binding protein. Protein structures were obtained from the RCSB Protein Data Bank. All protein residues are shown in a blue cartoon preset with cysteine residues labelled in red cartoon, respectively, using PyMOL software.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyHomo Sapiens